What SeqPal Can Do For You
Diverse Next Generation Sequencing(NGS) Services
Targeted Sequencing
Focus on sequencing specific regions of the genome rather than the entire genome. These panels consist of a selection of genes or genomic regions that are known to be relevant to a particular disease, condition, or research question. It is cost-effective, and provides increased sensitivity.
Metagenomics 16S ITS
Analyze the genetic diversity of microbial communities in environmental samples, with 16S being employed for bacteria and archaea, and ITS for fungi. These approaches provide valuable insights into the composition and structure of complex microbial populations within diverse ecosystems.
CRISPR Validation
Validate and confirm the on-target sequence changes introduced by CRISPR. This includes confirming insertions, deletions, or substitutions at the targeted genomic location. Also used to detect off-target sites.
Whole Genome Sequencing(WGS)
DNA sequencing involves determining the complete nucleotide sequence of an organism’s entire genome. Applications in Population Genetics, used as a reference, and it is essential to basic research.
Whole Exome Sequencing(WES)
By capturing and sequencing the protein-coding regions, researchers can identify and study variations, mutations, and other genetic changes that are associated with a wide range of genetic disorders and diseases.
RNA Sequencing
A method for high-throughput sequencing of RNA molecules, providing insights into gene expression and transcriptome analysis.
mRNA-seq
Analyze the transcriptome of a biological sample by sequencing messenger RNA (mRNA). Applications include gene expression profiling, identification of alternative splicing, differential gene expression analysis, and discovery of novel transcripts, among others.
miRNA-seq or Small RNA-seq
MicroRNAs are short RNA molecules, typically 19-25 nucleotides long, playing a crucial role in post-transcriptional regulation of gene expression. Applications include microRNA profiling, differential expression analysis, biomarker discovery, and more.
CHIP Sequencing(CHIP-Seq)
A powerful technique merging chromatin immunoprecipitation (CHIP) assays with sequencing to pinpoint genome-wide DNA binding sites. This method facilitates the comprehensive detection of interactions between proteins and nucleic acids on a genomic scale.
Single-Cell Sequencing
A revolutionary approach to analyzing DNA or RNA sequence information at the individual cell level. Wide variety of applications including below.
Single-Cell Gene Expression
Profile the gene expression of individual cells in a high-throughput manner. It provides insights into the transcriptional profiles of individual cells, allowing for the identification of cell types and the study of cell heterogeneity within tissues
Single-Cell Immune Profiling
Study the immune system at the single-cell level, characterizing immune cell populations, identifying specific immune cell subsets, and understanding their response to diseases and pathogens.
Single-Cell CNV (Copy Number Variation) Analysis
This application allows for the detection of copy number variations in single cells. It is valuable for understanding genomic instability in cancer cells and other diseases.
Single-Cell ATAC-Seq (Assay for Transposase-Accessible Chromatin with Sequencing)
This technique is used to study the chromatin accessibility of individual cells. It provides insights into the regulatory elements and epigenetic landscape of cells.
Single-Cell Multiome (Feature Barcode and V(D)J)
The Multiome application combines gene expression data with information on cell surface protein expression, cell-specific V(D)J (Variable, Diversity, Joining) immune receptor sequences, and other features. This enables a comprehensive view of cellular function and diversity.
Long-Read Nanopore Sequencing
Utilize nanopore-based sequencing platforms to generate long sequences of DNA or RNA. Long-read nanopore sequencing is well-suited for de novo genome assembly. It also suitable for identifying and characterizing structural variations in the genome and full-length RNA transcripts, plasmid verification.
Methyl Sequencing
Examine methylation patterns within a genome. These patterns are pivotal in gene regulation, cellular development, and diverse biological processes. Applications include epigenetic regulation studies, differential DNA methylation analysis, biomarker discovery, and more.
ATAC Sequencing
ATAC(Assay for Transposase-Accessible Chromatin) Sequencing is a molecular biology technique that assesses chromatin accessibility, revealing open genomic regions associated with regulatory elements. Through transposition, library preparation, and high-throughput sequencing, ATAC-seq provides researchers with high-resolution insights into chromatin structure, aiding the study of gene regulation, development, and disease processes. Its efficiency and sensitivity make ATAC-seq a widely adopted method in genomics research.
Complete GENOMICS
Sequencing on the Complete GENOMICS
What You Will Receive From SeqPal
Quality-Controlled Library Prep
SeqPal offers library preparation services, and to ensure high-quality sequencing results.
SeqPal conducts thorough quality checks for each sample before and/or after the library preparation process. Quality-control testings includes:
DNA Concentration & Purity
Measures DNA concentration using spectrophotometer and/or fluorometer.
DNA / RNA Integrity
Analyzes the size distribution of DNA, RNA, and sequencing library using Bioanalyzer.
Library Concentration
Precisely measures sequencing library concentration using Real-Time PCR (qPCR).
Sequencing Report
Each sequencing report from SeqPal includes raw data presented in the FASTQ format.
Bioinformatics Analysis Report
SeqPal offers optional enhanced report using QIIME pipeline.
Sample bioinformatic analysis listed below:
- Bacteria 16S Metagenomics & Fungi 18S/ITS Metagenomics
- Rank-abundance curve
- Taxonomic classification
- Rarefaction curve
- Principal coordinate analysis
- Hierarchical clustering
- Shotgun Metagenomics
- Taxon classification of reads across all ranks
- Microbial abundance estimation across all ranks
- Cross-sample comparisons across all ranks
- Dynamic, visual exploration of microbial taxonomy
- Interactive, searchable data tables
- Functional analysis
- Disease Gene Panels
- Variant discoveries
- Antibody Sequencing
- Frequency analysis
- CRISPR Sequencing
- sgRNA knockout analysis
- CHIP Sequencing
- Peakcalling
Sequencing Platforms
Illumina Systems
Chemistry & Flow Cell Specification
| Sequencer and flowcell | Read length | Output(paired-end reads)/flowcell |
|---|---|---|
| NovaSeq 6000 S4 | up to 2×150 bp | 16 – 20 billion |
| NovaSeq 6000 S2 | up to 2×150 bp | 6.6 – 8.2 billion |
| NovaSeq 6000 S1 | up to 2×150 bp | 2.6 – 3.2 billion |
| NovaSeq 6000 SP | up to 2×250 bp | 1.3 – 1.6 billion |
| NextSeq 2000 P3 | up to 2×150 bp | 2.4 billion |
| NextSeq 2000 P2 | up to 2×300 bp | 800 million |
| NextSeq 2000 P1 | up to 2×300 bp | 200 million |
| MiSeq V3 | up to 2×300 bp | 50 million |
| MiSeq V2 | up to 2×250 bp | 30 million |
| MiSeq Micro | up to 2×150 bp | 4 million |
| MiSeq Nano | up to 2×250 bp | 2 million |
| Sequencer and flowcell | Read length | Output(paired-end reads)/flowcell |