What WE do

Next Generation Sequencing
Services

Explore the Sequencing Revolution with SeqPal – Your Genomic Maestro! We offer expert Next-Gen Sequencing (NGS) services, from library prep to cutting-edge sequencing and in-depth bioinformatics.

Tailor your genomics journey with SeqPal, letting us handle complexities while you focus on groundbreaking solutions. Elevate your genomics game with expertise, innovation, and personalized precision – choose SeqPal!

More about us

WHAT SEQPAL CAN DO

NGS Services

Targeted Sequencing

Focused genome sequencing with selected gene panels provides cost-effective analysis and heightened sensitivity for disease-related research.

Metagenomics 16S ITS

Using 16S for bacteria and archaea, and ITS for fungi, the genetic diversity of microbial communities in environmental samples is analyzed to gain valuable insights into their composition and structure across diverse ecosystems.

CRISPR validation

CRISPR Validation

Confirm and validate CRISPR-induced sequence modifications at the target genomic location, identifying insertions, deletions, substitutions, and assessing potential off-target sites.

Whole Genome Sequencing (WGS)

Whole Genome Sequencing(WGS)

DNA sequencing determines an organism’s complete nucleotide sequence, serving in Population Genetics, as a reference, and playing an essential role in basic research.

Whole Exome Sequencing (WES)

Whole Exome Sequencing(WES)

Focus on capturing and sequencing protein-coding regions to identify and study variations, mutations, and genetic changes linked to various genetic disorders and diseases.

RNA Sequencing

A high-throughput technique that sequences RNA molecules, offering valuable information on gene expression and transcriptome analysis.

CHIP Sequencing

Chip Sequencing

Combine chromatin immunoprecipitation assays with sequencing to precisely locate DNA binding sites across the genome, facilitating the comprehensive analysis of interactions between proteins and nucleic acids.

Single-cell Sequencing

Single-Cell Sequencing

Examine DNA or RNA sequence information at the individual cell level, with a wide range of applications.

Long-read Nanopore Sequencing

Long-Read Nanopore Sequencing

Use nanopore-based platforms for extended DNA/RNA sequences, suitable for de novo genome assembly, identifying structural variations, and full-length RNA transcript characterization, including plasmid verification.

Methyl sequencing

Methyl Sequencing

Examine genome methylation patterns, crucial for gene regulation and cellular development. Applications include epigenetic studies, differential DNA methylation analysis, and biomarker discovery.

ATAC Sequencing

ATAC sequencing identifies accessible genomic regions associated with regulatory elements, facilitating high-resolution insights into gene regulation and disease processes in genomics research.

Complete GENOMICS

Sequencing on the Complete GENOMICS

Why Seqpal

SeqPal Advantage

Tailored workflows to meet your specific needs

Quality Library Prep

SeqPal provides quality-controlled library preparation services utilizing kits from leading brands.

Affordable Services

Affordable, high-quality NGS services partner in San Francisco Bay Area.

Quick Turnaround Time

Submit your libraries and receive a bioinformatics report in as little as 3-5 days.

FAQs

What if my sample concentration is lower than the recommended concentration?

Different library prep kits and sequencer systems have varied minimum requirements. Please contact us, info@seqpal.com, to discuss the minimum amount of material needed for your specific kit or system.

Are you able to perform DNA or RNA extraction?

Certainly! We offer DNA and RNA extraction services. Reach out to us to discuss your sample type and requirements.

Is there any other information that needs to be sent before sending the samples?

DNA can be shipped in ambient conditions, while RNA requires shipment in dry ice for optimal quality. Please adhere to these guidelines for sample transportation.

What kit or library preparation protocol is used?

We primarily use Illumina’s kits for DNA-seq and mRNA-seq. However, we can accommodate other vendors’ kits upon request. Feel free to contact us for more information on library preparation.

What primers are used for 16S sequencing?

We use primers for the V3 and V4 hypervariable regions of 16S rRNA. If you have specific primer or region requirements, contact us to discuss custom protocols.

How many reads do I need for my samples?

The number of reads required varies based on your sample type and research goals. Here are general guidelines:

Whole Genome Sequencing: Aim for 30X coverage.
RNA-seq:
- Small Genomes: 5-10 million reads per sample.
- Human or Mouse: Target 20-30 million reads per sample.
- De novo transcriptome assembly projects may require 100 million reads per sample.
Whole Exome Sequencing: Optimal coverage is in the range of 100X-200X.
Bisulfite Sequencing: Aim for 30X coverage.

These guidelines serve as a starting point, and we recommend reaching out to us for a more personalized assessment based on your specific sample characteristics and research objectives.

How do I calculate coverage?

Coverage is calculated using the formula: Coverage = (read length) x (total number of reads) / (genome size). For example, 30x coverage of the human genome requires 600 million reads of 150 bp.

What if my sequencing needs do not fill up a lane or a flow cell?

If your sequencing requirements do not utilize the full capacity of a lane or flow cell, we offer the option to pool your libraries or samples with compatible ones. Please get in touch with us to discuss and arrange the appropriate pooling strategy that suits your needs.

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Next Generation SequencingServices